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In this pathway, Gly I uses GSH as a cofactor and conjugates MG with GSH to form S -D-lactoylglutathione (SLG), Gly II, and then produce d-lactate breaking SLG, and regenerate GSH (Figure 9) [1], thus playing important interaction with glyoxalase system

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The reduction of sulfate to sulfide occurs in three steps beginning with its conversion to adenosine 5'-phosphosulfate (APS)