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Importantly, these phenotypes contrast with the non-inducible liver-specific Gclc KO mouse strain ( Gclc f/f Albumin-Cre), which develops liver failure and dies shortly after birth 30

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Cartilage: Has no direct blood supply Relies on diffusion for nutrient delivery Contains relatively few cells (chondrocytes) Has low regenerative capacity Chondrocytes are responsible for: Producing collagen (primarily type II) Producing proteoglycans such as aggrecan Maintaining extracellular matrix structure When chondrocytes become dysfunctional due to inflammation, mechanical stress, or aging, cartilage degradation accelerates