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Immunohistochemical staining After dewaxing, rehydration, and antigen retrieval, tissue sections were treated with 3% H 2 O 2 to block endogenous peroxidase activity and then blocked with 5% goat serum

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Unlike standard sterile water for research which contains no preservative and must be used immediately after opening, bacteriostatic water maintains sterility through multiple punctures, making it ideal for laboratory applications requiring frequent use or multi-application protocols