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No difference was seen in the source of serum (SMD = −1.24, 95% CI: −2.79 to 0.31, and p = 0.117), plasma (SMD = −0.05, 95% CI: −1.43 to 1.34, and p = 0.948), erythrocyte (SMD = −0.97, 95% CI: −1.94 to 0.00, and p = 0.050), or blister fluid (SMD = −0.29, 95% CI: −1.56 to 0.98, and p = 0.657) (Figure 3(a))

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Immunofluorescence showed increased Iba1 + microglia with a shift from ramified to amoeboid morphology, and elevated CD68 + /Iba1 + co-localization in PND mice (Fig