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Equal amounts of protein (~60 μg) were subjected to SDS-PAGE (10%) and were, then, transferred to PVDF membranes, and membranes were blocked with 5% skim milk powder and incubated in a shaker at room temperature for 4 h and, then, membranes were incubated with the primary antibodies (rabbit PTEN, Cat

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Proper implementation of these research protocols requires careful attention to dosage precision, safety monitoring, and data collection methodologies