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When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 × 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

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doi: 10.1074/jbc.273.47.31075